Translational_Unit
NeoR/KanR

Part:BBa_K5088750:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-08-10)


TU neomycin/kanamycin resistance casette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 73
    Illegal EcoRI site found at 1484
    Illegal XbaI site found at 1207
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 73
    Illegal EcoRI site found at 1484
    Illegal NheI site found at 278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 73
    Illegal EcoRI site found at 1484
    Illegal BamHI site found at 79
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 73
    Illegal EcoRI site found at 1484
    Illegal XbaI site found at 1207
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 73
    Illegal EcoRI site found at 1484
    Illegal XbaI site found at 1207
    Illegal NgoMIV site found at 1029
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 878
    Illegal SapI.rc site found at 1088


Design Notes

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Source

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References